Fluorescent biosensor imaging

Expertise

Monitoring and quantitative analysis of signaling dynamics via existing/optimized or newly developed genetically encoded reporters and FRET-based biosensors in (single) living cells and tissues. (more information on the Laboratory webpage)

Instrumentation

Wide-field Nikon TiE inverted microscope with:

• Objectives: 10x and 20x (0.5 NA)
• Illumination: Lumencor Spectra X LED Light Engine
• Emission Filter sets and Dichroics (Chroma Technology) on automated filter wheel Lambda 10-B Smart Shutter (Sutter Instrument)
• Camera : DS-Qi2 CMOS camera (Nikon), 4908 x 3264 pixels
• OkoLab topstage incubation for CO2 and temperature control
• Software: Nikon NIS-Elements with JOBS module (Nikon)

Details Equipment configuration 

Location

VIB/UGent Center for Inflammation Research (IRC)

Room W278

Technologiepark-Zwijnaarde 71

9052 Gent-Zwijnaarde

Contact information

Prof. Dr. F. Riquet: franck.riquet@irc.vib-ugent.be